Guided Cryo-lamellae Preparation Application Note Download

“Cryogenic electron tomography (cryo-ET) is emerging as a powerful technique to acquire high-resolution 3D structures such as intracellular organelles and protein complexes in their near-native cellular environment. Biological samples thicker than about 500 nm are no longer electron-transparent and thus need thinning. To create such a thin section the use of a focused ion beam with a scanning electron microscope (FIB/SEM) has become the gold standard.”