Thermal degradation of a perovskite solar cell
Stream In situ TEM Liquid + biasing or heating
Stream is the number one solution for in situ liquid phase electron microscopy (LPEM). The plug-and-play system provides researchers with the capability to conduct high resolution TEM imaging and spectroscopy of nanomaterials, soft materials, or biological samples in dynamic liquid environments.
With the possibility to apply heating or biasing stimuli within the liquid environment, and even wider range of exciting phenomena can be investigated.
- High experimental success rate – microfluidic channel ensures sample-liquid interaction, flushes away bubbles, and maintains supply of fresh solution
- Full experimental control – accurately define speed, pressure, and direction of liquid flow
- Clean and repeatable – modular design makes cleaning easy, avoids cross-contamination and clogging
Stream key features
Dissolve bubbles, control liquid layer thickness, and flow direction via pressure-driven flow.
Modular sample holder avoids cross-contamination and clogging, easy to clean and upgrade.
Integrated Liquid Supply System provides inert gas purging, ease-of-use, and reproducibility.
Stream’s patented Nano-Cell innovates over the previous generation of in situ liquid holders that rely on diffusion-controlled “bathtub” designs. A combination of a dual-chip MEMS assembly with a spacer creates a microfluidic channel with an electron-transparent window encapsulating the viewing area. This is the only system giving the microscopist full control over the liquid-sample interaction.
Mass transport control
By varying inlet and outlet pressures, the user can precisely determine when liquid will be introduced and removed from the region of interest. Additionally, the liquid thickness can be minimized via flow control making it possible to collect high quality TEM data.
Reduce electron beam effects and radiolysis
One of the biggest concerns for life science applications is the harmful impact of water radiolysis on biological specimens. With optional graphene window layers, free radical species are scavenging thus minimizing beam damage to sensitive material
Reduced setup time
Older generation in situ liquid holders, like DENSsolutions Ocean, employ syringe pumps and rely on diffusion for liquid to reach the viewing area meaning it can take anywhere from 15 to 45 minutes to start an LPEM experiment. With Stream, the combination of the Nano-Cell and Liquid Supply System enables rapid liquid transport to the viewing area on the order of tens of seconds.
Stream Nano-Cell Specifications
|Liquid Heating||Liquid Biasing|
|Resolution||≤ 3 Å|
|Liquid thickness control||Yes, via inlet and outlet liquid pressure control|
|Liquid modes||Static or Flow (infusion, withdrawal)|
|Liquid flow measurement||Yes, via inlet flow meter|
|Liquid pressure safety limit||Yes|
|Temperature range||RT to ≤ 100°C||NA|
|Electrodes||4-point probe based microheater||3|
|Voltage range||NA||-10 V to +10V|
|Current range||NA||From pA to mA|
|AC impedance frequency range||NA||10 uHz to 1 MHz|
ThermoFisher Scientific (FEI)
|Fluidic Control||Integrated pressure-based pumps (Liquid Supply System)|
|Modular holder design||Yes|
|Modes||TEM, STEM, EDS, EELS, electron diffraction|
|Alpha tilt range||Up to ± 21° depending on polepiece||Up to ± 35° depending on polepiece|
Liquid Supply System (LSS)
The LSS contains everything needed to control in situ experiments with unmatched flexibility. Designed for ease-of-use, repeatability, and maximum versatility, the LSS greatly simplifies complex in situ TEM experiments to maximize valuable time at the microscope.
Ease of use
A flexible arm can situate comfortably within TEM workstations while the mobile base enables easy relocation and storage.
Inert gas purging
With the added capability of inert gas purging, excess liquid can be quickly removed allowing high resolution images, diffraction patterns, and EELS/EDS spectra to be acquired.
Reliable and reproducible results
Actively measure liquid flow via the liquid flow meter, enabling results to be compared across experiments.
Modular Sample Holder
The sample holder is the platform that connects the Liquid Supply System to the Nano-Cell. Machined with the highest precision fabrication techniques, the sample holder fits perfectly into the TEM’s goniometer and maintains mechanical stability and ultra-vacuum performance during use.
A removable and modular sample holder tip makes for easy assembly, cleaning, and replacement of components. This not only ensures clean experiments and eliminates cross-contamination but makes training and maintenance much easier to manage.
Replaceable tubing material
The tubing within the holder can be easily removed for cleaning and reinserted within minutes – ensuring a clean experiment every time. In cases where flushing the tubing is not effective, a fresh set of tubing can also be replaced.
Simply by replacing the bottom chip of the Nano-Cell with a different MEMS configuration, Stream can be upgraded to enable Liquid + Biasing or Liquid + Heating functionality.
- Materials synthesis and growth
- Soft material
- Brownian single particle analysis
- Protein-protein interaction analysis
- Organic crystal growth
- Vesicle formation and liposome formation