The Air Water Interface and Sample Preparation for Cryo-EM
The air-water interface (AWI) is essentially the chaotic boundary where a solution meets the air. When protein molecules in solution contact the AWI, they tend to align themselves along the interface with the hydrophilic regions facing inward towards the water environment and the hydrophobic regions facing outwards towards the air environment. The AWI presents one […]
How The Ceres Clean Station and Vitri-lock Are Making Dry Rooms Obsolete
Cryogenic electron tomography (cryo-ET) provides detailed 3D structural information of unique cellular landscapes making it a crucial technique for gaining a complete understanding of the disease state, drug interactions, and other biological processes. However, the sample preparation process associated with generating samples for cryo-ET is extremely difficult and time-consuming. Typical sample yields, even for skilled […]
Minimize Ice Contamination During Cryogenic Lamella Milling Using CERES Ice Shield from Delmic
Cryogenic electron tomography (cryo-ET) provides the unprecedented ability to study biological matter in its native state at sub-nanometer resolution. Cryo-electron tomograms are 3D reconstructions from 2D images collected using a cryogenic transmission electron microscope (cryo-TEM) over a range of orientations. A notable advantage of the technique is that the sample preparation workflow produces specimens unperturbed […]
Producing High-quality Lamella For Cryo Electron Tomography with Integrated Cryo-CLEM
Cryogenic electron tomography (cryo-ET) is a powerful technique that is used to obtain 3D volumes of macromolecular structures– such as cells, bacteria, or organelles – in their native environment at sub-nanometer resolution [1]. A series of 2D transmission electron microscope (TEM) images are acquired over a range of tilts relative to the electron beam and […]
The Impact of Ice Contamination on Cryo-Electron Microscopy
Cryogenic electron microscopy (cryo-EM) is an advanced high-resolution imaging technique used to gain insight into the smallest structures and interactions within biological materials in their native states. Specifically, cryogenic electron tomography (cryo-ET) is capable of generating 3D reconstructions, from thin lamella extracted from whole cells, at sub-nanometer resolution by collecting a series of 2D images […]