Electrospinning Image Gallery

This backscattered electron micrograph shows the effect on the microstructure of Polycaprolactone (PCL) electrospun fibers when a low voltage (-5 kV) is used on the collector in a Fluidnatek LE-100. Since the fibers have more attraction towards the collector (when compared to a grounded collector) they have less time to stretch during the process, creating the curly pattern throughout the sample. Fiber-fiber bonding between fibers is also increased as the solvent does not have enough time to fully evaporate.

Closer look at electrospun polyacrylonitrile (PAN) fibers made using the Fluidnatek LE-50. These PAN fibers have a standard deviation less than 50 nm as they were developed under tight environmental conditions. These results can be obtained all year round when temperature and relative humidity and tightly controlled with the Fluidnatek technology.

Electron micrograph of electrospun polyacrylonitrile (PAN) made using the Fluidnatek LE-100. These fibers were collected on top of a polyethylene with carbon black substrate to allow for easy sample removal. PAN fibers are typically used for filtration, energy storage applications and to coat medical devices.

Backscattered electron micrograph of electrospun polycaprolactone (PCL) made using the Fluidnatek LE-500. To achieve reproducibility and fiber consistency, the electrospinning conditions were set to 24 ± 1°C and 40 ± 3%. These randomly oriented fibers offer a pore size around 20 µm to allow for cell infiltration for tissue engineering related applications.

This SEM image shows electrospun polycaprolactone (PCL) made in a Fluidnatek LE-50 at 1,000 rpm and at a translational needle speed of 20 mm/s and a low voltage of -4 kV. The combination of slow translational speed, along with the high rpm and low voltage, allowed the fibers to be collected with a peculiar wavy pattern with significant amount of fiber-fiber bonding that can increase mechanical properties of the final sample.

Microstructure of an electrospun nerve conduit made out of electrospun polylactic acid (PLA) made in the Fluidnatek LE-100. Characteristic features of this sample include the perpendicular aligned PLA fibers on the central portion of the sample (right side of the image) and randomly oriented PLA fibers surrounding the aligned fibers. The packing density of the aligned layers can be controlled with the Fluidnatek technology to fine tune final sample.

Micrograph of electrospun polybutylene succinate (PBS), a biodegradable polymer with water and carbon dioxide byproducts upon degradation by microorganisms. These plant based fibers were electrospun with the Fluidnatek LE-50 and are currently being used for single use food packaging and related applications.

Electron micrograph of biodegradable beaded fibers made out of polycaprolactone (PCL) using the Fluidnatek LE-100 with a 5 mm outside diameter mandrel rotating at 200 rpm. This image was acquired by mixing the signal of the backscatter and secondary electron detectors in a 1:1 ratio. In the past, this type of fiber morphology was thought to be a disadvantage in the electrospinning field. These days the beaded fiber structure is used for drug encapsulation, coat medical devices, increase efficiency of air filtration, among others.

Scanning electron microscope image showing the microstructure of electrospun fiberglass made with a Fluidnatek LE-50. Electrospun fiberglass is being used as a flame retardant material in different applications including textiles. These fibers could also be used as heat dissipation to protect sensitive materials from fire hazards.

Electron micrograph of biodegradable porous polycaprolactone (PCL) with a diameter around 10 µm. This image shows a close up onto the porous structure of the electrospun PCL fibers. The nanoscale porosity was achieved thanks to the quick solvent evaporation during the electrospinning process.

Microstructure of porous polycaprolactone (PCL) fibers with a diameter around 10 µm. These fibers were produced in a single step with the Fluidnatek LE-50 using a single solvent. These large fibers have good elongation properties, making it an ideal material to be used for artificial blood vessels, especially to mimic the tunica media layer of the saphenous vein. The nanoscale porosity was achieved thanks to the quick solvent evaporation during the electrospinning process.

This micrograph shows the cross-section of electrospun polylactide acid (PLA) made using a Spinbox equipment at 1,000 rpm. Thanks to the high glass transition temperature of PLA, the sample preparation to visualize the cross-section only required a simple cut with a scalpel. Other polymers need to be freeze fractured to prevent smearing from happening. Electrospun PLA fibers are typically used for wound healing and drug delivery applications, along with coating medical devices.

SEM image of electrospun polycaprolactone (PCL) made using the Fluidnatek LE-50. This image was acquired with a backscatter electron detector at an accelerating voltage of 5 kV. The microstructure reveals fiber-fiber bonding. This is due to low collector voltage during sample processing. When fibers are attracted faster to the collector, the solvent has less time to evaporate, allowing fibers to be wet and generate fiber-fiber bonding.

Coronary artery stent with an outside diameter of 5 mm coated with electrospun polycaprolactone (PCL). This SEM image is a composite of 5 rows and 8 columns stitched automatically to have a representative look at the microstructure of the coated surface. Stents are coated with fibers to make them more biocompatible. The beauty of the electrospinning technique is that it allows stents to be coated with different polymeric materials and drugs can be incorporated to improve patient healing

Backscattered electron micrograph of electrosprayed polycaprolactone (PCL) particles made with a Fluidnatek LE-50 and collected onto a liquid reservoir. These electrosprayed particles are characterized by their collapsed structure and the nanoscale porosity, both due to the use of a low boiling point and high vapor pressure solvent, along with their large diameter (>20 µm). These PCL particles can be used for drug delivery applications and to uniformly coat medical devices

Electron micrograph of electrosprayed polycaprolactone (PCL) microparticles made with a Fluidnatek LE-50. These particles were made with a high boiling point and low vapor pressure solvent allowing them to obtain a smooth and rounded morphology. After collecting onto a liquid reservoir, the media was evaporated, and the PCL particles agglomerated for future experiments

These electrosprayed polycaprolactone (PCL) rough microparticles were made on a single step using a Spinbox equipment and collected onto a flat plate collector covered with aluminum foil. Note the two distinctive surfaces (Janus morphology) on the right particle. Increasing the surface area of a particle, especially without post-processing and at room temperature, can help with drug delivery applications by loading thermal sensitive materials

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 0 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 50 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At this speed the fibers are still randomly oriented throughout the sample structure.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 200 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At 200 rpm the PCL fibers are still randomly oriented throughout the sample structure.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 500 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At 500 rpm the PCL fibers are still randomly oriented, but some initial reorientation can be seen throughout its microstructure.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 1,000 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At 1,000 rpm the PCL fibers realign during collection and the pore size of the sample is slightly increasing at the same time.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 1,500 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At 1,500 rpm the PCL fibers are realigning towards 90 degrees.

Randomly oriented fibers made out of polycaprolactone (PCL) and on top of a 10 cm diameter drum in a Spinbox equipment. These fibers were collected at 2,000 rpm to study the effect of fiber orientation under the influence of only the rotational speed in the drum collector. At 2,000 rpm the PCL fibers are aligned towards 90 degrees and the fiber diameter has been slightly decreased due to the high linear speed during collection. This orientation is used to increase mechanical properties and biomimic native extracellular matrix of different types of tissue.

Stitched image of 40 scanning electron micrographs of polycaprolactone (PCL) fibers collected on top of a 1 cm diameter rod at 200 rpm and with the collector grounded in a Fluidnatek LE-100 equipment. At 0 kV on the collector a rough microstructural surface is observed due to residual charge, causing fibers to localize in certain areas of the sample, affecting the final porosity of the electrospun material. By using a higher bias this effect will be completely removed and the final sample will contain a smooth surface.

Backscattered electron micrograph of polycaprolactone (PCL) fibers collected on top of a 1 cm diameter rod at 200 rpm in a Fluidnatek LE-100 equipment. At 200 rpm, and for large deposition times, a rough microstructural surface is observed that is due to residual charge, causing fibers to localize in certain areas of the sample, affecting the final porosity of the electrospun material. This microstructural effect is removed when using higher linear speeds or when combined with a higher bias at the same rotational speed.

This SEM image shows the microstructure of ribbon-shaped gelatin fibers made out of electrospinning with a Fluidnatek LE-50 and collected onto a rotating drum. The ribbon-shape structure is typically observed when the solvent evaporates fast during the spinning process. The fast evaporation process causes the fibers to collapse and obtain this flattened structure. A rounded morphology can be obtained by optimizing solution properties by using a high boiling point solvent like acetic acid.

Backscattered electron micrograph of electrospun nylon 6,6 (N66) fibers made with a Fluidnatek LE-500 with a multi-needle system and continuously collected onto a roll-to-roll collector. N66 is a commonly used polyester for air and liquid filtration applications due to its hydrophilicity, good solvent resistance, tailored fiber diameter, pore size tuning and ease of scalability with the electrospinning technology.

Micrograph of electrospun Nylon nanofibers made with a Fluidnatek LE-100 and collected onto a flat plate collector. Nylon nanofibers are commonly used for filtration applications, tissue engineering like bone regeneration when incorporated with hydroxyapatite, and medical sutures as they are stronger and durable when compared with silk.

The effect of fiber deposition density and microstructure was studied using electrospun polycaprolactone (PCL) fibers, collecting onto a flat plate collector of a Fluidnatek LE-500 by having all processing parameters constant and only changing the collector voltage. With a grounded collector (0 kV) we can see a low fiber density and large pore size.

The effect of fiber deposition density and microstructure was studied using electrospun polycaprolactone (PCL) fibers, collecting onto a flat plate collector of a Fluidnatek LE-500 by having all processing parameters constant and only changing the collector voltage. With a -1 kV applied in the collector, we can see a denser deposition when compared to the 0 kV sample. All fibers look relatively dry at these processing conditions.

The effect of fiber deposition density and microstructure was studied using electrospun polycaprolactone (PCL) fibers, collecting onto a flat plate collector of a Fluidnatek LE-500 by having all processing parameters constant and only changing the collector voltage. With a -5 kV applied in the collector, we can see a denser deposition when compared to the -1 kV sample. All fibers look relatively dry at these processing conditions.

The effect of fiber deposition density and microstructure was studied using electrospun polycaprolactone (PCL) fibers, collecting onto a flat plate collector of a Fluidnatek LE-500 by having all processing parameters constant and only changing the collector voltage. With a -10 kV applied in the collector, we can see that the fibers are not fully dried as there is more fiber-fiber contact, and the pore size has been reduced.

Backscattered electron micrograph of electrospun polycaprolactone (PCL) fibers, collecting onto a flat plate collector of a Fluidnatek LE-500 with a collector voltage of -10 kV. Fiber-fiber bonding is clearly seen throughout the microstructure. Although typically considered a non-desired defect, these wet fibers can be used to adhere two layers of materials with the electrospinning technique. Dry fibers can be obtained after adhesion by simply changing the collector voltage towards 0 kV.

Electron micrograph of electrosprayed polycaprolactone (PCL) microparticles made with a Fluidnatek LE-50. These particles have a diameter around 4 µm and were collected on top of polyethylene with carbon black to allow ease of removal post-processing. This smooth surface can be made with other types of polymers to obtain dry particles in a single step, encapsulate materials, and increase throughput by scaling up the electrospraying process for industrial production.

This SEM image shows an electrosprayed polycaprolactone (PCL) microparticles made with a Fluidnatek LE-50 using a low boiling point and high vapor pressure solvent. These particles show a characteristic collapsed surface with pores throughout its diameter ranging from a couple microns to the nanoscale size.

Microstructure of a sample made by combining electrospun polycaprolactone (PCL) nanofibers and electrosprayed PCL microparticles using the Fluidnatek LE-100. Applications like tissue engineering can benefit from combining fibers and particles in a single step and at room temperature conditions to have encapsulated growth factors that can be released at specific times. These types of samples can also be used for drug delivery applications on medical devices.

This SEM image shows the microstructure of polycaprolactone (PCL) nanofibers when combined with PCL electrosprayed particles at the same time in the Fluidnatek LE-100. To achieve this, the sample was collected onto a rotating drum by electrospinning the fibers in a horizontal setup and electrospraying the particles in a vertical setup, both needles pointing towards the same collector.

Microstructure of electrospun polylactic acid (PLA) fibers with sub-micron diameter. These fibers were produced in a single step with the Fluidnatek LE-50 using a solvent mixture of dichloromethane and dimethyl formamide solvent. PLA is used in FDA approved medical products, making it an ideal material to be used for artificial blood vessels, covering stent or for wound healing processes.

Electrospun collagen fibers using 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) as the solvent. Collagen is commonly used in wound healing applications as it is a polymer found in the in vivo environment. This sample was developed using the Fluidnatek LE-50 and under tight environmental conditions (23°C and 60% relative humidity) to allow for ease of processability.

Fiberglass is typically use in applications were durability and heat resistance is a must. These electrospun beaded fibers were made with the Fluidnatek LE-50 using the rotating collector platform to generate a sample of 20 cm x 32 cm.

Microstructure of polycaprolactone (PCL) microfibers with a diameter around 8 µm. These fibers were produced with dichloromethane (DCM). Since DCM has a low boiling point and high vapor pressure, it evaporates fast during sample processing, generating large fibers. The final sample has large pore size, allowing the sample to be an ideal candidate for tissue engineering applications as cells will be able to infiltrate and proliferate in the material

Upon close inspection, we see a porous structure on these 8 µm polycaprolactone (PCL) fibers. The small porosity allows cells to anchor and proliferate easier. These fibers were made using the Fluidnatek LE-50 using a rotating drum collector of 10 cm in diameter at 200 rpm.

Electrospinning allows the use of blending materials during sample processing. This image shows electrospun microscale fibers composed of polycaprolactone (PCL) and Nylon 6,6 (N66). Before processing, these two polymers were blended with the same solvent. During processing the final material is a combination of PCL:N66 that will impart properties to fulfill different application needs.

This microstructure shows microscale fibers of blended polycaprolactone (PCL) and polyethylene terephthalate (PET). The combination of PCL and PET is typically used when mechanical properties on the final structure are needed.

This SEM image taken on the Phenom Desktop SEM shows the microstructure of blended polycaprolactone and polyethylene terephthalate microfibers. These PCL:PET fibers are also combined when the degradation rate of PCL needs to be fine-tuned by adding a biostable PET, or when PET fibers need to be in combination with a biodegradable polymer like PCL

Post-processing as-spun electrospun fibers is typically done when microstructure or properties of the final sample are needed. This image shows the microstructure changes of PCL:PET fibers when treated with acetone for 5 seconds. At a magnification of 2,500 X the microstructure looks similar to the as-spun PCL:PET fibers.

Upon close inspection we can see smaller pores on the surface of the fibers when the sample is imaged at 5,000 X. This porosity could change microstructure properties, drug release properties, or even cell proliferation depending on application needs

Microstructure of acetone post-treated microfibers of PCL:PET. Imaging at 10,000 X we can clearly see the nanoscale porosity that has appeared on the surface of the electrospun fibers. Depending on the polymer compatibility, blended ratio and interaction during processing, different type of post-processing structures could be obtained and fine tuned based on application needs.

Microstructure of microscale polylactic acid (PLA) fibers with an average diameter of 6 µm. These fibers were produced in a single step with the Fluidnatek LE-50 using a single solvent. These large fibers have good elongation properties, making it an ideal material to be used for artificial blood vessels, especially to mimic the tunica media layer of the saphenous vein.

Electron micrograph of biodegradable porous polylactic acid (PLA) with an average diameter of 6 µm made with dichloromethane (DCM) as the solvent in solution. This image reveals a porous structure of the surface of the electrospun PLA fibers. The nanoscale porosity was achieved thanks to the quick solvent evaporation during the electrospinning process.

Microstructure of Nylon 6,6 (N66) nanoscale fibers with trapped pollen on its surface. Nylon 6,6 is commonly used for filtration applications. Electrospinning allows the generation of nanoscale N66 fibers allowing for easy mechanical entrapment of particles on filtration media.

Electrospun micrograph showing trapped pollen on the nanoscale fibers of Nylon 6,6 (N66) made with electrospinning. N66 is an easy to process polymer through the electrospinning technique. It has been used in scale up processes to generate more than 1,000 m2/h of material.

This image shows an overall look on the filtration media made out of Nylon 6,6 with trapped pollen on its structure. Electrospun fibers allow for mechanical filtration and samples that can be reused when compared to statical filtration media.

The microstructure of thermoplastic polyurethane (TPU) fibers generated with the Fluidnatek LE-50 using a mandrel collector of 5 mm in diameter. TPU fibers are commonly used in medical application as it is a flexible polymer with good mechanical properties. The electrospinning technique allows for fine tuning the fiber diameter and pore size to fulfill different application needs.

Electron micrograph of electrospun thermoplastic polyurethane (TPU) fibers. This image shows a combination of micron and sub-micron scale TPU fibers made with the electrospinning technique. TPU is a polymer used in medical product currently approved and cleared by the Food and Drug Administration (FDA).

Overall view of a surgical mesh coated with electrospun thermoplastic polyurethane (TPU) fibers. TPU fibers were used to coat the surface of the surgical mesh to make it more biocompatible by allowing the structure to resemble the extracellular matrix. By using electrospinning, the addition of fibers can make the surgical mesh more biocompatible and accepted by the body after implantation.

This stitched SEM image shows the microstructure of TPU fibers when electrospun on top of a surgical mesh for a long time. The ability of electrospinning to coat different substrates with different thickness give the flexibility to obtain desired properties based on application needs.

This image shows the back area of the surgical mesh coated with electrospun TPU fibers. Electrospinning has the ability to properly attach electrospun fibers to any type of mesh and prevent delamination. This allows the final sample to not go over post-processing needed with typical techniques used by the scientific community.

Microstructure image of electrospun hyaluronic acid (HA), a natural polymer typically used in food packaging, cosmetics, and tissue engineering applications. This image with a magnification of 10,000 X reveals sub-micron fibers. The analysis was performed with a mixture of backscattered and secondary detector at a ratio of 50:50.

This image shows the microstructure of electrospun nanoscale polyethylene oxide (PEO) fibers. These fibers were developed with the Fluidnatek LE-50 using a rotating drum of 10 cm in diameter and rotating at 100 rpm. The solvent used for processing PEO was water. To prevent needle clogging or dryness, the environmental conditions were maintained at 28°C and 40% relative humidity.

Electron micrograph of electrospun polyethylene terephthalate (PET) beaded fibers. Beaded fibers are usually not desired by the scientific community. Meanwhile, beaded fibers offer advantages like encapsulation ability of different active ingredients or drugs that can be delivered without bursting release. In order to prevent beaded fibers in the final morphology, the easy solution is to increase the concentration of the final solution. This will allow you to obtain only fiber formation without beads.

This SEM image shows the microstructure of nanoscale electrospun fibers out of polyvinylidene fluoride (PVDF). Nanoscale fibers are commonly developed with the electrospinning technique, and we can develop them with a diameter less than 20 nm depending on solution properties. These fibers were made with the Fluidnatek LE-50 using the rotating drum of 10 cm in diameter and at 50 rpm to obtain randomized morphology.