BioNavis MP-SPR
MP-SPR Navi™ 410A KAURIS
4 channel system for cell based assays
The MP-SPR Navi™ 410A KAURIS is a fully automated, four-channel Multi-Parametric Surface Plasmon Resonance (MP-SPR) system designed for high-sensitivity, real-time analysis of molecular interactions. By simultaneously monitoring four independent flow channels on a single sensor, the system increases experimental throughput while delivering highly reproducible results. Equipped with two laser wavelengths, the Navi™ 410A KAURIS provides precise characterization of binding kinetics, adsorption processes, and thin film properties, making it a powerful platform for life science research, biomolecular interaction studies, and advanced surface characterization.
4 Channels, 2 Wavelengths
Simultaneously measure in 4 channels on one sensor. Use up to 2 different wavelengths in each channel
Automation
Fully automated system with integrated degassing routines and Kinetic Titration function allows for measurements from 7 samples.
Versatile
Study proteins, viruses, lipid nanoparticles, bacteria and living cells in physiologically relevant media.
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4 channel system for cell based assays
The MP-SPR Navi™ 410A KAURIS is a fully-automated Multi-Parametric Surface Plasmon Resonance (MP-SPR) system specifically engineered for live cell analysis, cell adhesion studies, and cell-based interaction measurements under physiologically relevant conditions. Unlike conventional SPR systems, the MP-SPR Navi™ 410A KAURIS captures the complete SPR curve, providing comprehensive information for characterizing cell attachment, spreading, morphology, and cell-surface interactions in real-time.
The system features four independent fluidic channels, enabling the simultaneous analysis of four experiments on a single sensor surface for increased throughput and experimental consistency. An elastomer-coated prism simplifies sensor handling and allows cells to be cultured ex situ before analysis, with the same sensor subsequently characterized using complementary techniques such as atomic force microscopy (AFM) and optical microscopy.
Designed for advanced life science research, the MP-SPR Navi™ 410A KAURIS provides precise control of temperature, flow rate, shear stress, and culture medium composition, allowing researchers to closely replicate physiological conditions. Its exceptionally wide 40°–78° angular measurement range enables reliable measurements in cell culture media as well as other complex biological samples, including serum and saliva, making it an ideal platform for applications in drug discovery, tissue engineering, biomaterials, immunology, and cancer research.
Application Areas:
MP-SPR Navi 410A KAURIS
Product Features
Angular scanning mode
The MP-SPR Navi™ 410A KAURIS employs a true goniometric Surface Plasmon Resonance (SPR) configuration with a rotating detector to perform angular scanning, known as MP-SPR mode. Instead of measuring at a single angle, the system scans across a wide angular range of 40° to 78°, capturing the complete SPR curve with an exceptional angular resolution of 0.001°. This comprehensive measurement provides access to multiple analytical parameters beyond those available with conventional SPR systems, enabling more detailed characterization of molecular interactions and thin films. Typical sampling rates are less than two seconds, depending on the selected angular range and resolution, allowing rapid, real-time monitoring of dynamic surface processes.
Laser Wavelengths
The MP-SPR Navi™ 410A KAURIS is equipped with a 670 nm laser and 785 nm laser as standard, enabling high-sensitivity measurements across both flow channels. The additional laser wavelengths provide multi-wavelength MP-SPR measurements that enable the detection of molecular conformational changes and the characterization of thin film properties, including layer thickness, refractive index, and structural evolution, extending the system beyond traditional interaction analysis. This feature is critical in applications involving layer characterization, live cell studies, and extracellular vesicle size and concentration analysis.
Liquid handling
The MP-SPR Navi™ 410A KAURIS features an automated liquid handling system designed to increase throughput, improve reproducibility, and enable unattended MP-SPR experiments. The system accommodates up to 7 different samples for sequential automated analysis and features 4 independent flow channels with software-controlled selection of serial or parallel sample injections.
Buffer delivery is precisely controlled using high-accuracy syringe pumps and an integrated degasser, ensuring stable, pulse-free flow conditions for reliable kinetic measurements. The system also includes a dual loop injection mode enabling fast KineticTitration experiments. The system supports flow rates from 1 µL/min to 1,000 µL/min, providing the flexibility required for a wide range of biomolecular interaction studies, surface characterization experiments, and assay development workflows.
Flow cells
The MP-SPR Navi™ 410A KAURIS is equipped with a standard four-channel PDMS flow cell with a low internal volume of 1 µL/channel, minimizing sample consumption while enabling rapid exchange of solutions. To support a wide range of research applications, the system is compatible with several optional flow cells, including cell culture flow cells and custom flow cell designs to meet specialized experimental requirements.
Kinetic Titrations
MP-SPR enables label-free, real-time measurement of biomolecular interactions, providing quantitative determination of binding affinity (KD) and association (ka) and dissociation (kd) rate constants. Using a continuous flow injection system, analytes interact with surface-immobilized ligands while the instrument monitors binding and dissociation in real-time. Advanced analysis software supports multiple kinetic models, allowing accurate characterization of interactions involving proteins, antibodies, peptides, small molecules, and other biomolecules.
To maximize throughput, MP-SPR supports KineticTitration, a measurement approach that eliminates regeneration steps between successive analyte concentrations. By injecting samples sequentially from low to high concentration and measuring dissociation only after the final injection, KineticTitration can reduce total experiment time by up to 50% while improving assay efficiency and preserving ligand activity.
PureKinetics™
PureKinetics™ is a unique MP-SPR feature that accurately separates true molecular binding from changes caused by differences in sample and buffer composition, commonly known as the bulk effect. Unlike conventional SPR systems, which require extensive bulk calibration and a dedicated reference channel, MP-SPR captures the complete SPR curve and uses multiple optical parameters to correct for bulk refractive index changes in real-time.
This inline bulk correction improves the accuracy and reliability of kinetic and affinity measurements, even in challenging experimental conditions. PureKinetics™ is particularly valuable for analyzing crude biological samples, experiments containing high concentrations of solvents such as DMSO, and applications involving lipid bilayers, biomaterials, and other surfaces where creating an appropriate reference channel is difficult or impossible. By eliminating the need for a reference channel, PureKinetics™ simplifies assay development while providing more reliable interaction data.
MP-SPR Navi 410A KAURIS
Sensors & Substrates
MP-SPR Sensors
The MP-SPR Navi™ 410A KAURIS supports a broad selection of sensor surfaces to accommodate diverse application requirements. Available sensor coatings include metals such as gold (Au), silver (Ag), copper (Cu), and platinum (Pt); inorganic materials including silicon dioxide (SiO₂), aluminum oxide (Al₂O₃), and titanium dioxide (TiO₂); as well as functionalized surfaces such as carboxymethyl dextran (CMD) and Ni²⁺-modified sensors for biomolecular interaction studies.
The system’s sensor holder also supports ex situ surface preparation, allowing researchers to deposit custom thin films or coatings using techniques such as spin coating before measurement. This flexibility enables the characterization of user-defined materials and expands the range of applications in surface science, biomaterials, coatings, and nanomaterials research.
Unique Regenerable Avidin – Kit
Revolutionizing regenerable avidin kit allows reversible binding of biotinylated ligands and thus same sensor slide can be used to measure interaction of multiple ligand molecules.
MP-SPR Navi 410A KAURIS
Applications
Molecular Interactions
Multi-Parametric Surface Plasmon Resonance (MP-SPR) is a highly sensitive, label-free technology for studying molecular interactions across a broad range of analytes, including proteins, peptides, nucleic acids, small-molecule drugs, viruses, nanoparticles, living cells, and bacteria. The technique provides accurate, real-time measurements of binding affinity, kinetic rate constants, and bound mass for interactions such as antibody-antigen, receptor-ligand, and drug-target binding. Unlike conventional SPR, MP-SPR can directly analyze interactions in complex biological matrices, including 100% serum, plasma, saliva, and other physiologically relevant fluids. Its unique PureKinetics™ technology uses complete SPR curve measurements to continuously correct for changes in the bulk refractive index (bulk effect) within each measurement channel. This real-time correction eliminates the need for a dedicated reference channel for bulk compensation, improving the accuracy of kinetic measurements in crude samples. MP-SPR also enables the characterization of membrane-associated interactions by accurately measuring binding events involving liposomes, membrane extracts, and living cells. This capability allows researchers to investigate biomolecular interactions under conditions that closely mimic their native biological environment.
Live Cells
Multi-Parametric Surface Plasmon Resonance (MP-SPR) enables analysis of live cell interactions, providing biologically relevant information while preserving the complexity of living cell models. This capability makes MP-SPR a powerful tool for applications in drug discovery, regenerative medicine, biosensor development, cancer research, immunology, and point-of-care diagnostics. By monitoring cellular responses as they occur, MP-SPR allows researchers to investigate nanoparticle uptake kinetics, drug absorption pathways, receptor activation (including GPCR signaling), and cell activation profiles. The technique can also be used to determine half-maximal effective concentration (EC₅₀), evaluate drug efficacy, and study the mechanisms by which nanoparticles, viruses, and other therapeutic agents enter cells under physiologically relevant conditions. MP-SPR is equally valuable for characterizing cell-surface interactions. By using functionalized sensor surfaces, researchers can quantify cell adhesion kinetics, receptor-mediated binding affinity, and binding specificity of different cell populations. These measurements support the development and optimization of biomaterials, tissue engineering scaffolds, medical device coatings, and antifouling or antimicrobial surfaces, providing critical insights into cellular responses at the material interface.
Biocompatibility
Multi-Parametric Surface Plasmon Resonance (MP-SPR) enables label-free, real-time characterization of biomolecular interactions in clinically relevant complex media, including serum, plasma, urine, bacterial suspensions, and cell culture media. This capability makes MP-SPR particularly valuable for biocompatibility and biointerface research, including the evaluation of antifouling coatings for medical devices and characterization of protein corona formation on nanoparticle drug carriers. Unlike conventional SPR, where changes in sample composition can produce significant bulk refractive index effects, MP-SPR simultaneously measures multiple optical parameters through its unique goniometric optical configuration. Using PureKinetics™, these parameters are correlated in real-time to correct for bulk effects, enabling accurate kinetic analysis even in complex biological samples.
Nanoparticles
Multi-Parametric Surface Plasmon Resonance (MP-SPR) enables label-free, real-time characterization of nanoparticle interactions while preserving the native properties of the nanoparticles. It provides highly sensitive measurements of nanoparticle binding kinetics, drug release, and interactions with biological targets, making it an ideal tool for developing nanoparticle-based drug delivery systems and contrast agents. By delivering quantitative, real-time data under biologically relevant conditions, MP-SPR helps researchers optimize nanoparticle formulations, improve targeting performance, and accelerate nanomedicine research.